Efeito antiglicativo da folha de oliveira (olea europaea) contra produtos finais de glicação avançada da dieta em ratos
Palavras-chave:
Produtos finais de glicação avançada, Expressão gênica, Glicação, İnflamação, Folha de oliveira.Resumo
Objetivo
O objetivo era examinar o efeito antiglicação do Extrato de Folha de Oliveira (EFO) em ratos alimentados com uma dieta rica em Advanced Glycation End Products (AGEs, Produtos Finais de Glicação Avançada).
Métodos
Os ratos foram divididos em seis grupos: um grupo dieta padrão, um grupo dieta padrão + 500 mg/kg/semana de EFO, um grupo dieta padrão + 1000 mg/kg/semana de EFO, um grupo dieta H-AGEs, um grupo dieta H-AGEs + 500 mg/kg/semana de EFO e um grupo dieta H-AGEs + 1000 mg/kg/semana de EFO.
Resultados
Após uma intervenção de 8 semanas, as quantidades séricas de AGE e receptor de AGE (RAGE) de ratos no grupo High Advanced Glycation End Products (HAGE, Níveis elevados de produtos finais de glicação avançada) foram maiores do que no grupo controle (p<0,001). A quantidade sérica de AGE de ratos no grupo HAGE foi significativamente maior do que aqueles que receberam suplementação de EFO de 1000 mg/kg/semana (p<0,001). A suplementação de EFO de 1000 mg/kg/semana foi eficaz em marcadores inflamatórios no soro. As alterações histopatológicas no fígado foram reduzidas pela suplementação de EFO. No entanto, não afetou significativamente as expressões gênicas de Receptor Advanced Glycation End Products (RAGE, receptores de produtos finais de glicação avançada) e fator nuclear kappa B (NF-ĸB) do fígado.
Conclusão
Esta pesquisa revelou que o EFO pode ser um agente fitoterápico essencial na nutrição sustentável, pois é um resíduo vegetal e um potencial agente antiglicação.
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Referências
1. Semba RD, Bandinelli S, Sun K, Guralnik JM, Ferrucci L. Relationship of an advanced glycation end product, plasma carboxymethyl-lysine, with slow walking speed in older adults: the InCHIANTI study. Eur J Appl Physiol. 2010;108(1):191-5.
2. Liu H, Zhang R, Wang W, Xia X, Xu Z, Xiang X. Inhibitory effects and mechanisms of phenolic compounds in rapeseed oil on advanced glycation end product formation in chemical and cellular models in vitro. Food Chem. 2024;447:139056.
3. Demirer B, Yardımcı H, Erem Basmaz S. Inflammation level in type 2 diabetes is associated with dietary advanced glycation end products, Mediterranean diet adherence and oxidative balance score: A pathway analysis. J Diabetes Complications. 2023;37(1):108354.
4. Guo WA, Davidson BA, Ottosen J, Ohtake PJ, Raghavendran K, Mullan BA, Dayton MT, Knight PR. Effect of high advanced glycation end-product diet on pulmonary inflammatory response and pulmonary function following gastric aspiration. Shock. 2012;38(6):677-84.
5. Hegab Z, Gibbons S, Neyses L, Mamas MA. Role of advanced glycation end products in cardiovascular disease. World J Cardiol. 2012;4(4):90-102.
6. Litwinowicz K, Waszczuk E, Gamian A. Advanced Glycation End-Products in Common Non-Infectious Liver Diseases: Systematic Review and Meta-Analysis. Nutrients. 2021;13(10).
7. Nowotny K, Jung T, Höhn A, Weber D, Grune T. Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. Biomolecules. 2015;5(1):194-222.
8. Rungratanawanich W, Qu Y, Wang X, Essa MM, Song BJ. Advanced glycation end products (AGEs) and other adducts in aging-related diseases and alcohol-mediated tissue injury. Exp Mol Med. 2021;53(2):168-88.
9. Kobori A, Miyashita M, Miyano Y, Suzuki K, Toriumi K, Niizato K, et al. Advanced glycation end products and cognitive impairment in schizophrenia. Plos One. 2021;16(5):e0251283.
10. Venter C, Pickett K, Starling A, Maslin K, Smith PK, Palumbo MP, et al. Advanced glycation end product intake during pregnancy and offspring allergy outcomes: A Prospective cohort study. Clin Exp Allergy. 2021;51(11):1459-70.
11. Tian Z, Chen S, Shi Y, Wang P, Wu Y, Li G. Dietary advanced glycation end products (dAGEs): An insight between modern diet and health. Food Chemistry. 2023;415:135735.
12. Chen G. Dietary N-epsilon-carboxymethyllysine as for a major glycotoxin in foods: A review. Compr Rev Food Sci Food Saf. 2021;20(5):4931-49.
13. Uribarri J, Woodruff S, Goodman S, Cai W, Chen X, Pyzik R, et al. Advanced glycation end products in foods and a practical guide to their reduction in the diet. J Am Diet Assoc. 2010;110(6):911-16.e12.
14. Asif A, Zeeshan N, Mehmood S. Antioxidant and antiglycation activities of traditional plants and identification of bioactive compounds from extracts of Hordeum vulgare by LC-MS and GC-MS. J Food Biochem. 2020;44(9):e13381.
15. Khan M, Liu H, Wang J, Sun B.Inhibitory effect of phenolic compounds and plant extracts on the formation of advance glycation end products: A comprehensive review. Food Research International. 2020;130:108933.
16. Koch ER, Deo P. Nutritional supplements modulate fluorescent protein-bound advanced glycation endproducts and digestive enzymes related to type 2 diabetes mellitus. BMC Complement Altern Med. 2016;16(1):338.
17. Kontogianni VG, Charisiadis P, Margianni E, Lamari FN, Gerothanassis IP, Tzakos AG. Olive leaf extracts are a natural source of advanced glycation end product inhibitors. J Med Food. 2013;16(9):817-22.
18. Vijaykrishnaraj M, Wang K. Dietary natural products as a potential inhibitor towards advanced glycation end products and hyperglycemic complications: A phytotherapy approaches. Biomed Pharmacother. 2021;144:112336.
19. Song Q, Liu J, Dong L, Wang X, Zhang X. Novel advances in inhibiting advanced glycation end product formation using natural compounds. iomed Pharmacother. 2021;140:111750.
20. Spagnuolo L, Della Posta S, Fanali C, Dugo L, De Gara L. Antioxidant and Antiglycation Effects of Polyphenol Compounds Extracted from Hazelnut Skin on Advanced Glycation End-Products (AGEs) Formation. Antioxidants (Basel). 2021;10(3):424.
21. Jia W, Ma R, Zhang R, Fan Z, Shi L. Synthetic-free compounds as the potential glycation inhibitors performed in in vitro chemical models: Molecular mechanisms and structure requirements. Trends Food Sci. Technol. 2022;128:147-59.
22. Asgharpour Dil F, Ranjkesh Z, Goodarzi MT. A systematic review of antiglycation medicinal plants. Diabetes Metab Syndr. 2019;13(2):1225-29.
23. Gürbüz M, Öğüt S. Potential Health Benefits of Olive Leaf. Turkiye Klinikleri J Health Sci. 2018;3(3):242-53.
24. Omar SH. Oleuropein in Olive and its Pharmacological Effects. Sci Pharm. 2010;78(2):133-54.
25. Morelló JR, Vuorela S, Romero MP, Motilva MJ, Heinonen M. Antioxidant activity of olive pulp and olive oil phenolic compounds of the arbequina cultivar. J Agric Food Chem. 2005;53(6):2002-8.
26. Alirezaei M, Dezfoulian O, Neamati S, Rashidipour M, Tanideh N, Kheradmand A. Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats. J Physiol Biochem. 2012;68(4):583-92.
27. Barbaro B, Toietta G, Maggio R, Arciello M, Tarocchi M, Galli A, et al. Effects of the Olive-Derived Polyphenol Oleuropein on Human Health. Int J Mol Sci. 2014;15(10):18508-24.
28. Janahmadi Z, Nekooeian AA, Moaref AR. Oleuropein Offers Cardioprotection in Rats with Acute Myocardial Infarction. Cardiovasc Toxicol. 2015;15(1):61-8.
29. Muellenbach EA, Diehl CJ, Teachey MK, Lindborg KA, Archuleta TL, Harrell NB, et al. Interactions of the advanced glycation end product inhibitor pyridoxamine and the antioxidant alpha-lipoic acid on insulin resistance in the obese Zucker rat. Metabolism. 2008;57(10):1465-72.
30. Zhang X, Xu L, Chen W, Yu X, Shen L, Huang Y. Pyridoxamine alleviates mechanical allodynia by suppressing the spinal receptor for advanced glycation end product-nuclear factor-κB/extracellular signal-regulated kinase signaling pathway in diabetic rats. Mol Pain. 2020;16:1744806920917251.
31. Csongová M, Renczés E, Šarayová V, Mihalovičová L, Janko J, Gurecká R, et al. Maternal Consumption of a Diet Rich in Maillard Reaction Products Accelerates Neurodevelopment in F1 and Sex-Dependently Affects Behavioral Phenotype in F2 Rat Offspring. Foods. 2019;8(5):168
32. Yang S, Zhou H, Wang G, Zhong XH, Shen QL, Zhang XJ, et al. Quercetin is protective against short-term dietary advanced glycation end products intake induced cognitive dysfunction in aged ICR mice. J Food Biochem. 2020;44(4):e13164.
33. Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. Faseb J. 2008;22(3):659-61.
34. Takata T, Sakasai-Sakai A, Takino JI, Takeuchi M. Evidence for Toxic Advanced Glycation End-Products Generated in the Normal Rat Liver. Nutrients. 2019;11(7):1612.
35. Yamagishi S, Matsui T. Role of receptor for advanced glycation end products (RAGE) in liver disease. Eur J Med Res. 2015;20(1):15.
36. Alves M, Calegari VC, Cunha DA, Saad MJ, Velloso LA, Rocha EM. Increased expression of advanced glycation end-products and their receptor, and activation of nuclear factor kappa-B in lacrimal glands of diabetic rats. Diabetologia. 2005;48:2675-81.
37. Tobon-Velasco JC, Cuevas E, Torres-Ramos MA. Receptor for AGEs (RAGE) as mediator of NF-kB pathway activation in neuroinflammation and oxidative stress. CNS Neurol Disord Drug Targets. 2014;13(9):1615-26.
38. Prasad K, Mishra M. AGE-RAGE Stress, Stressors, and Antistressors in Health and Disease. Int J Angiol. 2018;27(1):1-12.
39. Chen MC, Lin JA, Lin HT, Chen SY, Yen GC. Potential effect of advanced glycation end products (AGEs) on spermatogenesis and sperm quality in rodents. Food Funct. 2019;10(6):3324-33.
40. Wang J, Cai W, Yu J, Liu H, He S, Zhu L, et al. Dietary Advanced Glycation End Products Shift the Gut Microbiota Composition and Induce Insulin Resistance in Mice. Diabetes Metab Syndr Obes. 2022;15:427-37.
41. Silvestrini A, Giordani C, Bonacci S, Giuliani A, Ramini D, Matacchione G+ Anti-inflammatory effects of olive leaf extract and its bioactive compounds oleacin and oleuropein-aglycone on senescent endothelial and small airway epithelial cells. Antioxidants (Basel). 2023;12(8):1509.
42. Fayez N, Khalil W, Abdel-Sattar E, Abdel-Fattah AM. In vitro and in vivo assessment of the anti-inflammatory activity of olive leaf extract in rats. Inflammopharmacology. 2023;31(3):1529-38.
43. Asghari AA, Mahmoudabady M, Shabab S, Niazmand S. Anti-inflammatory, anti-oxidant and anti-apoptotic effects of olive leaf extract in cardiac tissue of diabetic rats. J Pharm Pharmacol. 2022;74(7):961-72.
44. Márquez K, Márquez N, Ávila F, Cruz N, Burgos-Edwards A, Pardo X, et al. Oleuropein-Enriched Extract From Olive Mill Leaves by Homogenizer-Assisted Extraction and Its Antioxidant and Antiglycating Activities. Front Nutr. 2022;9:895070.
45. Matsuda H, Wang T, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of protein glycation and radical scavenging activities. Bioorg Med Chem. 2003;11(24):5317-23.
46. Xie Y, Chen X. Structures required of polyphenols for inhibiting advanced glycation end products formation. Curr Drug Metab. 2013;14(4):414-31.
47. Palioura E, Palimeri S, Piperi C, Sakellariou S, Kandaraki E, Sergentanis T, et al. Impact of Androgen and Dietary Advanced Glycation End Products on Female Rat Liver. Cell Physiol Biochem. 2015;37(3):1134-46.
48. Poulsen MW, Andersen JM, Hedegaard RV, Madsen AN, Krath BN, Monošík R, et al. Short-term effects of dietary advanced glycation end products in rats. Br J Nutr. 2016;115(4):629-36.
49. Ashraf JM, Shahab U, Tabrez S, Lee EJ, Choi I, Ahmad S. Quercetin as a finer substitute to aminoguanidine in the inhibition of glycation products. International Int J Biol Macromol. 2015;77:188-92.
50. Li X, Zheng T, Sang S, Lishuang, Lv. Quercetin Inhibits Advanced Glycation End Product Formation by Trapping Methylglyoxal and Glyoxal. J. Agric. Food Chem. 2014;62(50):12152-58.
51. Vlassopoulos A, Lean MEJ, Combet E. Protein–phenolic interactions and inhibition of glycation – combining a systematic review and experimental models for enhanced physiological relevance. Food & Function. 2014;5(10):2646-55.
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Copyright (c) 2026 Büşra Demirer, Mehmet Özdemir, Gülhan Samur
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