BACTERIAL CONTAMINATION OF THE GUTTA-PERCHA CONES USED IN THE DENTISTRY CLINICS OF THE PONTIFÍCIA UNIVERSIDADE CATÓLICA DE CAMPINAS SCHOOL OF DENTISTRY
Keywords:
bacteria, decontamination, endodontics, Gutta-PerchaAbstract
Objective
To verify if there is bacterial contamination of the gutta-percha cones stored in two different ways in the dentistry c!inics of the Pontifícia Universidade Católica de Campinas School of Dentistry
Methods
ln this study, 39 gutta-percha cones were collected from two different storage means: sealed box (contrai group) and dry recipient and wet recipient (glycerin), from the Pontifícia Universidade Católica de Campinas. These were inoculated in Petri dishes in Brain Heart lnfusion and then were stored in anaerobiosis jars and placed in a 37°C incubator for 48 hours.
Results
After the incubation period of 24 and 48 hours, no bacterial growth was detected in the dishes.
Conclusion
Based on the results obtained in this research, the conclusion is that the gutta-percha cones, regardless of the storage means, seem to be free from contamination.
Downloads
References
Berger CR. Endodontia. São Paulo: Pancast; 1998. p.416-69.
Soler RM, Shocron ML. Endodoncia. Rosário: Lá Médica; 1957. p.305
lmura N, Zuolo ML. Endodontia para o clínico geral. São Paulo: Artes Médicas; 1998. p.73-102.
Cohen S, Burns, RC. Caminhos da polpa. 7.ed. Rio de Janeiro: Guanabara Koogan; 2000. p.243-341.
Estrela C, Figueiredo JAP. Endodontia princípios biológicos e mecânicos. São Paulo: Artes Médicas; 2001. p.657-96.
Moorer MR, Genet JM. Antibacterial activity of gutta-percha cones attributed to the zinc oxid compnet. Oral Surg Oral Méd Pathol. 1982; 53(5):508-17.
Lopes HP, Siqueira Jr JF. Endodontia: biologia e técnica. São Paulo: Médica e Científica; 1999. p.185-216, 427-84.
Friedman CE, Sandrik JL, Heur MA, Rap PGW. Composition and physical proprieties of gutta-percha endodontic filling materiais. J Endod. 1977; 3(8):304-8.
Moorer MR, Genet JM. Evidence for antibacterial activity of endodontic gutta-percha cones. Oral Surg Oral Méd Oral Pathol. 1982; 53(5):503-7
O Friedman CM, Dandrik JL, Hever MA, RAPP GW. Composition and mechanical properties of gutta-percha endodontic points. J Dent Res. 1975; 54(5):921-5.
Podbielski A, Boeckh C, Haller B. Growth inhibitory activity of gutta-percha points containing root canal medications os common endodontic bacterial pathogens as determined by na optimized in vitro assay. J Endod. 2000; 26(7):398-403
Cardoso CL, Kotaka CR, Redmerski R, Guilhermetti M, Queiroz AF. Rapid decontamination of gutta percha cones with sodium hypochlorite. J Endod. 1999; 25(7):498-501.
Gomes BPF, Ferraz CCR, Carvalho KC, Teixeira FB, Zaia AA, Souza-Filho FJ. Descontaminação química de cones de guta-percha por diferentes concentrações de Nacl. Rev APCD. 2001; 55(1):27-31.
Short RD, Dorn SO, Kuttler S. The crystallization of sodium hypochlorite on gutta-percha cones after the rapid-sterelization techinique: an sem study. J Endodontics. 2003; 29(10):670-3.
Kotaka CR, Redmerski R, Queiroz AF, Cardoso CL. Descontaminação rápida de cones de guta-percha na prática endodontica. Rev FOB. 1998; 6(2):73-80
Santos RB, Poisl MIP, Mattiello VS, Arruda FZ. Esterilidade dos cones de guta-percha, mito ou realidade? Rev RBO. 1999; 56(5):201-3
Salazar SJR, Silva JR, Siqueira AS, Avila MJC, Antoniazzi SJR. Avaliação in vitro da capacidade antimicrobiana de cones de guta-percha utilizados na obturação de canais radiculares. Rev Brás Odontol. 1995; 52(2):39-41.
Attin T, Zirkel C, Pelz K. Antibacterial properties if electron beam-sterilized gutta-percha cones. J Endodontics. 2001; 27(3):172-4.
Holland R, Barnabé PFE, Negata MSH, Mitsuda ST. Métodos de esterilização dos cones na endodoncia. Rev RGO. 1990; 30(2):133-7.
Leonardo MR, et. ai. Evaluation of the sterility and antimicrobial activity of gutta-percha cones. Braz Endod J. 1994; 73:16115-26
LopesHP, et ai. Analysis of the surfaces of gutta-percha cones after the chemical sterilization. Braz Endod J. 1997; 2(1):35-7
Leonardo MR, LEAL JM. Endodontia-tratamento de canais radiculares. 3.ed. São Paulo: Medicina Panamericana; 1998.
Namazikhah MS, Sullivan DM, Trnavsky GL. Gutta-percha: a look at the need for sterilization. J Calif Dent Assoe. 2000; 28(6):427-32.
Silva AS, Paiva JG, Antoniazzi JH. Avaliação da contaminação do cone de guta-percha durante o seu manuseio de ajuste para a obturação de canais radiculares. Rev Paul Odontol. 1988; 10(6):50-1.
Favero MS, Bond WW. Chemical desinfection of medical and surgical materiais. ln: Blockss: Desinfection, eterilization, and meservation 4thed. Philadelphya: Lea&Febiger. 1991; p.617-41.
Cardoso CL, Kotaka CR, Silve TC, Guilhermetti M, Tognim MCB. Esterilização rápida de cones de guta-percha por glutaraldeído. Rev APCD. 1997; 51(5):425-31.