Effect of caloric restriction on hepatic gamma-glutamyl transpeptidase activity and on glutathione levels
Keywords:
Gamma-glutamyltransferase, glutathione, protein-energy malnutrition, growth, pregnancy, lactationAbstract
The purpose of this study was to evaluate the effect of the acute (post-weaning growth) and chronic (lactating rats undernourished since prenatal period and their pups) caloric restriction on hepatic gamma-glutamyl transpeptidase activity and glutathione levels of rats. Diet consumption was either unrestricted (control group) or 30% restricted (undernourished group).Two trials, one of acute malnutrition and another of chronic malnutrition, were conducted. Acute restriction caused significant reduction in the weight gain of the growing rats, but chronically undernourished mothers showed a significantly higher weight gain/pup, since they presented fewer pups. No significant alterations in glutathione levels, gamma-glutamyl transpeptidase activity, or even in the hepatic protein concentration were observed under acute or chronic caloric restriction. The less pronounced unbalance in the profile of plasmatic amino acids and in the protein synthesis (consequence of caloric, rather than protein malnutrition) can justify such results.
Downloads
References
ÁLVAREZ-ORDÁS, I., GUITIÉRREZ, J.M., CASADO, C., FERNÁNDEZ, S., MENÉNDEZ-PATTERSON, A. Effect of maternal food restriction on the evolution of pregnancy in the rat. Revista Española de Fisiologia, Barcelona, v.48, n.4, p.277-284, 1992.
ATINMO, T., BALDIJÃO, C., POND, W.G., BARNES, R.H. The effect of dietary protein restriction on serum thyroxine levels of pregnant or growing swine. Journal of Nutrition, Philadelphia, v.108, n.9, p.1456-1533, 1978.
COMPORTI, M., MAELLARO, E., DEL BELLO, B., CASINI, A.F. Glutathione depletion: its effect on other antioxidant systems and hepatocelular damage. Xenobiotica, London, v.21, n.8, p.1067-1076, 1991.
COWARD, W.A., WHITEHEAD, R.G., LUNN, P.G. Reasons why hypoalbuminaemia may or may not appear in protein-energy malnutrition. British Journal of Nutrition, Cambridge, v.38, n.1, p.115-126, 1977.
DEAN, A.G., DEAN, J.A., BURTON, A.H., DICKER, R.C. Epi Info. Version 6. Atlanta : CDC/OMS, 1997. 586p.
FERRARI, F., GABRIELLI, P.R.M., MELLO, M.A.R. Restrição alimentar durante a gestação e suas implicações sobre o binômio mãe/feto: um modelo experimental utilizando ratas jovens e adultas. Alimentos e Nutrição, São Paulo, v.4, p.45-56, 1992.
GOSS, P.M., BRAY, T.M., NAGY, L.E. Regulation of hepatocyte glutathione by amino acid precursors and cAMP in protein - energy malnourished rats. Journal of Nutrition, Philadelphia, v.124, n.3, p.323-330, 1994.
HUM, S., KOSKI, K.G., HOFFER, L.J. Varied protein intake alters glutathione metabolism in rats. Journal of Nutrition, Philadelphia, v.122, n.10, p.2010-2018, 1992.
JACOBS, W.L.W. A colorimetric assay for γ- glutamyltranspeptidase. Clinica Chimica Acta, Amsterdam, v.31, p.175-179, 1971.
LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L., RANDALL, R.J. Protein measurement with the folin-phenol reagent. Journal of Biological Chemistry, Baltimore, v.193, n.1, p.265-275, 1951.
MEISTER, A. On the enzymology of amino transport. Science, New York, v.180, n.81, p.33-39, 1973.
PERSIJIN, J.P. et al. Colorimetric assay for γ-glutamyltranspeptidase. Clinica Chimica Acta, Amsterdam, v.35, p.239-240, 1971.
SAINZ, R.D., CALVERT, C.C., BALDWIN, R.L. Relationships among dietary protein feed intake and tissue protein turnover in lactating rats. Journal of Nutrition, Philadelphia, v.116, n.9, p.1820-1829, 1986.
SAVILLE, B. A scheme for the colorimetric determination of microgram amounts of thiols. Analyst, London, v.83, n.2, p.670-671, 1958.
SMITH, J.E., LUNN, P.G. Albumin-synthesizing capacity of hepatocytes isolated from rats fed diets differing in protein and energy content. Annals of Nutrition and Metabolism, Basel, v.28, n.5, p.281-287, 1984.
SZASZ, G. γ-glutamyltranspeptidase. In: BERGMEYER, H.U. (ed) Methods of enzymatic analysis. 2.ed. New York : Academic Press, 1974. v.2: p.715-719.
TATEISHI, N., HIGASHI, T., SHINYA, S., NARUSEA, A., SAKAMOTO, Y. Studies on the regulation of glutathione level in rat liver. Journal of Biochemistry, Tokyo, n.1, v.75, p.93-103, 1974.
TATEISHI, N. et al. Rat liver glutathione: possible role as a reservoir of cysteine. Journal of Nutrition, Philadelphia, v.107, n.1, p.51-61, 1977.
VIANNA DE OLIVEIRA, I.M., DE ANGELIS, R.C. Differential effects of protein restriction on gammaglutamyltranspeptidase [EC. 2.3.2.2] activity in young and mature rats. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v.29, n.2, p.213-217, 1996a.
VIANNA DE OLIVEIRA, I.M., FUJIMORI, E. Liver gamma-glutamyl trans-peptidase activity and glutathione levels in lactating rats and pups: effect of dietary protein quantity and feed intake. The Journal of Nutritional Biochemistry, Stoneham, v.7, n.2, p.93-98, 1996b.
VIANNA DE OLIVEIRA, I.M., DE ANGELIS, R.C., SILVA, L. Dietas à base de arroz e feijão aumentam a atividade plasmática e hepática da gama-glutamiltranspeptidase em ratos jovens. Archivos Latinoamericanos de Nutrición, Guatemala, 1998a. (No prelo).
VIANNA DE OLIVEIRA, I.M., SAKAUE, C., LIMA, A.R. Alterações na atividade da gama-glutamiltranspeptidase e nos níveis de glutationa em fígado de ratos submetidos a dietas deficientes em metionina. São Paulo : EEUSP, 1998b. 22p. (Resultados não publicados; Mimeografado).
VIANNA DE OLIVEIRA, FUJIMORI, E., PEREIRA, V.G., CASTRO, V.D. DL-methionine supplementation of rice and beans diets affects gamma-glutamytranspeptidase activity and glutathione content in livers of growing rats. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v.32, n.4, p.483-488, 1999.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Ida Maria VIANNA DE OLIVEIRA, Roseane Harue Yoshida de PAULO, Elizabeth FUJIMORI
This work is licensed under a Creative Commons Attribution 4.0 International License.
