Effects of acute restraint stress, chronic swim stress and glutamine administration on the release of superoxide from alveolar macrophages of rats
Keywords:
macrophages, alveolar, superoxide anion, stress, swimming, glutamineAbstract
Objective
To assess the release of superoxide anion from alveolar macrophages of rats submitted or not to acute restraint stress, forced swimming and glutamine supplementation.
Methods
Forty-two male Wistar rats aging roughly 62 days (standard deviation=3) were randomly divided into four groups: control, training, stress and glutamine. After the intervention, alveolar macrophages were collected and stimulated with phorbol myristate acetate to assess the release of superoxide anion.
Results
When compared with the first hour (control=26.2, standard deviation=4.2; training=28.7, standard deviation=5.1; stress=20.3 , standard deviation=4.4; glutamine=26.2, standard deviation=4.2), the release of superoxide increased (p<0.001) in all experimental groups in the second hour (control=38.4, standard deviation=4.9; training=40.7, standard deviation=6.1; stress=30.2, standard deviation=5.6; glutamine=39.2, standard deviation=5.2) of observation. Training and glutamine supplementation did not induce differences in the release of superoxide from alveolar macrophages when compared with the control group. Only the rats
submitted to stress showed a reduction in the release of superoxide in both the first (20.3, standard deviation=4.4; p<0.05) and second hours (30.2, standard deviation=5.6; p<0.05) of observation.
Conclusion
The results suggest that stress can be one of the factors associated with immunosuppression since reduced release of superoxide anion from macrophages can lead to reduced microbicidal capacity. On the other hand, the swimming protocol we used and the amount and route of glutamine supplementation did not change the release of superoxide from alveolar macrophages.
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Copyright (c) 2023 Elizabeth do NASCIMENTO, Carol Virgínia Góis LEANDRO, Marco Antônio Fidalgo AMORIM, América PALMEIRA, Taisy Cavalcante FERRO, Célia Maria Machado Barbosa de CASTRO, Raul Manhães de CASTRO
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