A single dose of dark chocolate increases parasympathetic modulation and heart rate variability in healthy subjects

Autores/as

  • Ana Amélia Machado DUARTE Universidade Federal de Ciências da Saúde de Porto Alegre
  • Cristiano MOSTARDA Universidade Federal do Maranhão
  • Maria Claudia IRIGOYEN Universidades de São Paulo
  • Katya RIGATTO Universidade Federal de Ciências da Saúde de Porto Alegre

Palabras clave:

Autonomic nervous system, Cocoa, Flavonoids

Resumen

Objective
The aim of this study was to investigate the acute effect of a single dose of dark chocolate (70% cocoa) on blood pressure and heart rate variability.

Methods
Thirty-one healthy subjects (aged 18-25 years; both sexes) were divided into two groups: 10 subjects in the white chocolate (7.4 g) group and 21 in the dark chocolate (10 g) group; measurements were performed at the university’s physiology lab. An electrocardiogram measured the sympathovagal balance by spectral and symbolic analysis.

Results
A single dose of dark chocolate significantly reduced systolic blood pressure and heart rate. After consuming 10 g of dark chocolate, significant increases were observed for heart rate variability, standard deviation of RR intervals standard deviation of all NN intervals, square root of the mean squared differences between adjacentnormal RR intervals root mean square of successive differences, and an increase in the high frequency component in absolute values, representing the parasympathetic modulation.

Conclusion
In conclusion the importance of our results lies in the magnitude of the response provoked by a single dose of cocoa. Just 10 g of cocoa triggered a significant increase in parasympathetic modulation and heart rate variability. These combined effects can potentially increase life expectancy because a reduction in heart rate variability is associated with several cardiovascular diseases and higher mortality. 

Citas

Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet. 1993; 342(8878):1007-11.

Manach C, Mazur A, Scalbert A. Polyphenols and prevention of cardiovascular diseases. Curr Opin Lipidol. 2005; 16(1):77-84.

Keli SO, Hertog MG, Feskens EJ, Kromhout D. Dietary flavonoids, antioxidant vitamins, and incidence of stroke: The Zutphen study. Arch Intern Med. 1996; 156(6):637-42.

Corti R, Flammer AJ, Hollenberg NK, Luscher TF. Cocoa and cardiovascular health. Circulation. 2009; 119(10):1433-41.

Buijsse B, Weikert C, Drogan D, Bergmann M, Boeing H. Chocolate consumption in relation to blood pressure and risk of cardiovascular disease in German adults. Eur Heart J. 2010; 31(13):1616-23.

Serafini M, Bugianesi R, Maiani G, Valtuena S, De Santis S, Crozier A. Plasma antioxidants from chocolate. Nature. 2003; 424(6952):1013.

Kondo K, Hirano R, Matsumoto A, Igarashi O, Itakura H. Inhibition of LDL oxidation by cocoa. Lancet. 1996; 348(9040):1514.

Baba S, Natsume M, Yasuda A, Nakamura Y, Tamura T, Osakabe N, et al. Plasma LDL and HDL cholesterol and oxidized LDL concentrations are altered in normo- and hypercholesterolemic humans after intake of different levels of cocoa powder. J Nutr. 2007; 137(6):1436-41.

Selmi C, Cocchi CA, Lanfredini M, Keen CL, Gershwin ME. Chocolate at heart: The anti inflammatory impact of cocoa flavanols. Mol Nutr Food Res. 2008; 52(11):1340-8.

Mao TK, Powell J, van de Water J, Keen CL, Schmitz HH, Hammerstone JF, et al. The effect of cocoa procyanidins on the transcription and secretion of interleukin 1 beta in peripheral blood mononuclear cells. Life Sci. 2000; 66(15):1377-86.

Bordeaux B, Yanek LR, Moy TF, White LW, Becker LC, Faraday N, et al. Casual chocolate consumption and inhibition of platelet function. Prev Cardiol. 2007; 10(4):175-80.

Persson IA, Persson K, Hagg S, Andersson RG. Effects of cocoa extract and dark chocolate on angiotensin-converting enzyme and nitric oxide in human endothelial cells and healthy volunteers-a nutrigenomics perspective. J Cardiovasc Pharmacol. 2011; 57(1):44-50.

Taubert D, Roesen R, Lehmann C, Jung N, Schomig E. Effects of low habitual cocoa intake on blood pressure and bioactive nitric oxide: A randomized controlled trial. JAMA. 2007; 298(1):49-60.

Vlachopoulos C, Aznaouridis K, Alexopoulos N, Economou E, Andreadou I, Stefanadis C. Effect of dark chocolate on arterial function in healthy individuals. Am J Hypertens. 2005; 18(6):785-91.

Desch S, Schmidt J, Kobler D, Sonnabend M, Eitel I, Sareban M, et al. Effect of cocoa products on blood pressure: Systematic review and meta-analysis. Am J Hypertens. 2010; 23(1):97-103.

Mehrinfar R, Frishman WH. Flavanol-rich cocoa: A cardioprotective nutraceutical. Cardiol Rev. 2008; 16(3):109-15.

Franchini KG, Cowley AW, Jr. Sensitivity of the renal medullary circulation to plasma vasopressin. Am J Physiol. 1996; 271(3Pt 2):R647-53.

Heart rate variability. Standards of measurement, physiological interpretation, and clinical use. Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology. Eur Heart J. 1996; 17(3):354-81.

Bertagnolli M, Schenkel PC, Campos C, Mostarda CT, Casarini DE, Bello-Klein A, et al. Exercise training reduces sympathetic modulation on cardiovascular system and cardiac oxidative stress in spontaneously hypertensive rats. Am J Hypertens. 2008; 21(11):1188-93.

Tobaldini E, Porta A, Wei SG, Zhang ZH, Francis J, Casali KR, et al. Symbolic analysis detects alterations of cardiac autonomic modulation in congestive heart failure rats. Auton Neurosci. 2009; 150(1-2):21-6.

Pumprla J, Howorka K, Groves D, Chester M, Nolan J. Functional assessment of heart rate variability: Physiological basis and practical applications. Int J Cardiol. 2002; 84(1):1-14.

Akita M, Kuwahara M, Itoh F, Nakano Y, Osakabe N, Kurosawa T, et al. Effects of cacao liquor polyphenols on cardiovascular and autonomic nervous functions in hypercholesterolaemic rabbits. Basic Clin Pharmacol Toxicol. 2008; 103(6):581-7.

Malpas SC. Sympathetic nervous system overactivity and its role in the development of cardiovascular disease. Physiol Rev. 2010; 90(2):513-57.

Minson CT, Halliwill JR, Young TM, Joyner MJ. Influence of the menstrual cycle on sympathetic activity, baroreflex sensitivity, and vascular transduction in young women. Circulation. 2000; 101(8):862-8.

Taubert D, Berkels R, Roesen R, Klaus W. Chocolate and blood pressure in elderly individuals with isolated systolic hypertension. JAMA. 2003; 290(8):1029-30.

Montano N, Porta A, Cogliati C, Costantino G, Tobaldini E, Casali KR, et al. Heart rate variability explored in the frequency domain: A tool to investigate the link between heart and behavior. Neurosci Biobehav Rev. 2009;33(2):71-80.

Porta A, Faes L, Masé M, D’Addio G, Pinna GD, Maestri R, et al. An integrated approach based on uniform quantization for the evaluation of complexity of short-term heart period variability: Application to 24 h Holter recordings in healthy and heart failure humans. Chaos. 2007; 17(1):015117.

Porta A, Tobaldini E, Guzzetti S, Furlan R, Montano N, Gnecchi-Ruscone T. Assessment of cardiac autonomic modulation during graded head-up tilt by symbolic analysis of heart rate variability. Am J Physiol Heart Circ Physiol. 2007; 293(1):H702-8.

Guzzetti S, Borroni E, Garbelli PE, Ceriani E, Della Bella P, Montano N, et al. Symbolic dynamics of heart rate variability: A probe to investigate cardiac autonomic modulation. Circulation. 2005;112(4):465-70.

Egan BM, Laken MA, Donovan JL, Woolson RF. Does dark chocolate have a role in the prevention and management of hypertension?: Commentary on the evidence. Hypertension. 2010; 55(6):1289-95.

Zomer E, Owen A, Magliano DJ, Liew D, Reid CM. The effectiveness and cost effectiveness of dark chocolate consumption as prevention therapy in people at high risk of cardiovascular disease: Best case scenario analysis using a Markov model. BMJ. 2012; 344:e3657.

Grassi D, Lippi C, Necozione S, Desideri G, Ferri C. Short-term administration of dark chocolate is followed by a significant increase in insulin sensitivity and a decrease in blood pressure in healthy persons. Am J Clin Nutr. 2005; 81(3):611-4.

Engler MB, Engler MM, Chen CY, Malloy MJ, Browne A, Chiu EY, et al. Flavonoid-rich dark chocolate improves endothelial function and increases plasma epicatechin concentrations in healthy adults. J Am Coll Nutr. 2004; 23(3):197-204.

Fisher ND, Hughes M, Gerhard-Herman M, Hollenberg NK. Flavanol-rich cocoa induces nitric oxide-dependent vasodilation in healthy humans. J Hypertens. 2003; 21(12):2281-6.

Ried K, Sullivan T, Fakler P, Frank OR, Stocks NP. Does chocolate reduce blood pressure? A meta analysis. BMC Med. 2010; 8:39.

Heiss C, Schroeter H, Balzer J, Kleinbongard P, Matern S, Sies H, et al. Endothelial function, nitric oxide, and cocoa flavanols. J Cardiovasc Pharmacol. 2006; 47 (Suppl 2:S128-35):S72-6.

Fraga CG, Actis-Goretta L, Ottaviani JI, Carrasquedo F, Lotito SB, Lazarus S, et al. Regular consumption of a flavanol-rich chocolate can improve oxidant stress in young soccer players. Clin Dev Immunol. 2005; 12(1):11-7.

Heiss C, Dejam A, Kleinbongard P, Schewe T, Sies H, Kelm M. Vascular effects of cocoa rich in flavan- -3-ols. JAMA. 2003; 290(8):1030-1.

Faridi Z, Njike VY, Dutta S, Ali A, Katz DL. Acute dark chocolate and cocoa ingestion and endothelial function: A randomized controlled crossover trial. Am J Clin Nutr. 2008; 88(1):58-63.

Loffredo L, Perri L, Catasca E, Pignatelli P, Brancorsini M, Nocella C, et al. Dark chocolate acutely improves walking autonomy in patients with peripheral artery disease. J Am Heart Assoc. 2014; 3(4):e001072. http://dx.doi.org/10.1161/JAHA.114.001072

Sudarma V, Sukmaniah S, Siregar P. Effect of dark chocolate on nitric oxide serum levels and bloodpressure in prehypertension subjects. Acta Med Indones. 2011; 43(4):224-8.

Flammer AJ, Hermann F, Sudano I, Spieker L, Hermann M, Cooper KA, et al. Dark chocolate improves coronary vasomotion and reduces platelet reactivity. Circulation. 2007; 116(21):2376-82.

Nurk E, Refsum H, Drevon CA, Tell GS, Nygaard HA, Engedal K, et al. Intake of flavonoid-rich wine, tea, and chocolate by elderly men and women is associated with better cognitive test performance. J Nutr. 2009; 139(1):120-7.

Letenneur L, Proust-Lima C, Le Gouge A, Dartigues JF, Barberger-Gateau P. Flavonoid intake and cognitive decline over a 10-year period. Am J Epidemiol. 2007; 165(12):1364-71.

Hollenberg NK, Fisher ND, McCullough ML. Flavanols, the Kuna, cocoa consumption, and nitric oxide. J Am Soc Hypertens. 2009; 3(2):105-12.

Tsuji H, Larson MG, Venditti FJ, Manders ES, Evans JC, Feldman CL, et al. Impact of reduced heart rate variability on risk for cardiac events. The Framingham Heart Study. Circulation. 1996; 94(11):2850-5.

Nolan J, Batin PD, Andrews R, Lindsay SJ, Brooksby P, Mullen M, et al. Prospective study of heart rate variability and mortality in chronic heart failure: Results of the United Kingdom heart failure evaluation and assessment of risk trial (UK-heart). Circulation. 1998; 98(15):1510-6.

Dekker JM, Crow RS, Folsom AR, Hannan PJ, Liao D, Swenne CA, et al. Low heart rate variability in a 2-minute rhythm strip predicts risk of coronary heart disease and mortality from several causes: The ARIC Study. Atherosclerosis Risk In Communities. Circulation. 2000; 102(11):1239-44.

Ajayi AAL, Hercule HC, Pamugo J, Dixon D, Oyekan AO. Interactions of the renin-angiotensin system and alpha-1 adrenoceptors on renal hemodynamics in healthy and acute renal failure rats: The role of nitric oxide. Blood Press. 2001; 10(4):238-46.

Kim M, Han CH, Lee MY. NADPH oxidase and the cardiovascular toxicity associated with smoking. Toxicol Res. 2014; 30(3):149-57.

Villarreal-Calderon R, Torres-Jardón R, Palacios Moreno J, Osnaya N, Pérez-Guillé B, Maronpot RR, et al. Urban air pollution targets the dorsal vagal complex and dark chocolate offers neuroprotection. Int J Toxicol. 2010; 29(6):604-15

Descargas

Publicado

2023-03-30

Cómo citar

Machado DUARTE, A. A. ., MOSTARDA, C. ., IRIGOYEN, M. C. ., & RIGATTO, K. . (2023). A single dose of dark chocolate increases parasympathetic modulation and heart rate variability in healthy subjects. Revista De Nutrição, 29(6). Recuperado a partir de https://puccampinas.emnuvens.com.br/nutricao/article/view/8129

Número

Sección

ARTIGOS ORIGINAIS