Action of ascorbic acid on the healing of malnourished rats’ skin wounds
Palabras clave:
Ascorbic acid, Malnutrition, Skin, Vitamins, Wound healingResumen
Objective
To evaluate the action of ascorbic acid on the healing of malnourished rats’ cutaneous wounds compared with normal weight rats.
Methods
We used 92 adult, male Wistar rats divided into four groups: 24 normal weight rats given only water and chow; 24 normal weight rats given vitamin C by gavage (340 mg/kg 12/12 hours); 22 malnourished rats given only water and chow; and 22 malnourished rats given vitamin C by gavage (340 mg/kg 12/12 hours). Malnutrition was induced by feeding the animals half of their daily energy requirement for 30 days. Two incisions were made, one sutured (healing by primary intention) and one left unsutured (healing by secondary intention). The rats were euthanized on the third, seventh, and fourteenth days of the experiment.
Results
The following parameters differed significantly between the groups (p>0.05): granulation of the wound edge in the primary and secondary intention; extent of injuries on day 7 for primary intention and on day 3 for secondary intention; reepithelialization on day 7 for primary intention; fibrin-leukocyte scab on day 14 forprimary intention; amount of neovascularization and concentration of macrophages, fibroblasts, and collagen fibers for primary and secondary intention.
Conclusion
The use of vitamin C in malnourished and normal weight rats increases fibroblast proliferation and collagen deposition in the tissue, which helps to improve healing both by primary and secondary intention.
Citas
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Derechos de autor 2023 Gisele Alborghetti NAI, Louise Maria Freitas MANZOLI, Tayane Carvalho Isidoro da SILVA, Larissa de Queiroz MAMEDE, Mary Ellen de Oliveira Martins DISCONZI, Rogério GIUFFRIDA
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
