Experimental diet based on the foods listed in the Family Budget Survey is more detrimental to growth than to the reflex development of rats
Palabras clave:
Diet, Growth, Lactation, RatsResumen
Objective
The present study assessed the pregnancy and lactation performances of rats fed an experimental diet based on the foods listed in the Family Budget Survey (Pesquisa de Orçamento Familiar) 2002/2003 and the impact of said diet on the growth and development of the pups until weaning.
Methods
Wistar (n=12) rats were randomly divided into two groups: a control group (control group, n=6) fed a commercial chow (Labina®, Brazil) and an experimental group (n=6) fed the Family Budget Survey diet during the entire pregnancy and lactation period. All animals had free access to food and water during the entire study period.
Results
The Family Budget Survey diet increased the duration of pregnancy (control group=21.00±0.00; POFG=21.57±0.55, p=0.025) and made the dams lose weight during the lactation period (control group=27.92±18.47g; POFG=-15.66±16.90g). The Family Budget Survey group presented low food, energy and nutrient intakes during pregnancy, which became even lower during lactation. Pups from Family Budget Survey dams presented lower body weight at weaning (control group=52.38±4.49g; POFG=39.88±2.78g,
p=0.001) and lower nose-to-anus length (control group= 117.37±0.64mm; POFG=125.62±0.96mm, p=0.001). However, some physical milestones and reflexes occurred earlier, such as the placing response reflex [control group= 12.00 (9.00-15.00) days; POFG=9.50 (9.00-14.00) days] aerial righting reflex [control group=18.00 (17.00-20.00) days; POFG=16.00 (13.00-18.00) days] and unfolding of the external ear [control group=3.00 (3.00-3.00) days; POFG=2.00 (2.00-3.00) days].
Conclusion
The Family Budget Survey diet seems to be more detrimental to the physical growth of the pups than to their brain growth, according to the assessed reflexes and physical milestones and measures. This may be due to the low protein content of the diet for rat reproduction and growth combined with adequate fat and essential fatty acid contents. Providing an adequate amount of protein to the Family Budget Survey diet may normalize pup growth.
Citas
National Research Council. Nutrient Requirements of Laboratory Animals. 4th ed. Washington: National Academy Press; 1995 [cited 2011 Jun 27]. Available from: <http://www.nap.edu/openbook.php?record_id=4758&page=154>.
Morgane PJ, Austin-LaFrance R, Bronzino J, Tonkiss J, Díaz-Cintra S, Cintra L, et al. Prenatal malnutrition and development of the brain. Neurosc Biobehav Reviews. 1993; 17:91-128. doi: 10.1016/S0149-7634(05)80234-9.
Bourre JM, Bonneil M, Clément M, Dumont O, Durand G, Lafont H, et al. Function of dietary polyunsaturated fatty acids in the nervous system PLEFA. 1993; 48:5-15. doi: 10.1016/0952-3278(93)90003-F.
Miller JP, German RZ. Protein malnutrition affects the growth trajectories of the craniofacial skeleton in rats. J Nutr. 1999 [cited 2011 Jul 10]; 129:2061. Available from: <http://jn.nutrition.org/content/129/11/2061.long>.
Cherala G, Shapiro BH, D’Mello AP. Two low protein diets differentially affect food consumption and reproductive performance in pregnant and lactating rats and long-term growth in their offspring. J Nutr. 2006 [cited 2011 Jul 10]; 136:2827-33. Available from: <http://jn.nutrition.
org/content/136/11/2827.full>.
Pine AP, Jessop NS. Maternal protein reserves and their influence on lactational performance in rats 3: The effects of dietary protein restriction and stage of lactation on milk composition. Br J Nutr. 1994 [cited 2011 Jul 10]; 72(6):815-30. Available from:
<http://journals.cambridge.org/abstract_S0007114594000887>.
Sampson DA, Hunsaker HA, Jansen GR. Dietary protein quality, protein quantity and food intake: Effects on lactation and on protein synthesis and tissue composition in mammary tissue and liver in rats. J Nutr. 1986; 116(3):365-75.
Moura EG, Lisboa PC, Custódio CM, Nunes MT, Souza KP, Passos MCF. Malnutrition during lactation changes growth hormone mRNA expression in offspring at weaning and in adulthood. J Nutrit Biochem. 2007; 18(2):134-9. doi: 10.1016/j.jnutbio. 2006.04.002.
Brito PD, Ramos CF, Passos MCF, Moura LG. Adaptive changes in thyroid function of female rats fed a high-fat and low-protein diet during gestation and lactation. Braz J Med Biol Res. 2006; 39(6):809-6. doi: 10.1590/S0100-879X2006000600015.
Torres N, Bautista CJ, Tovar AR, Ordáz G, RodríguezCruz M, Ortiz V, et al. Protein restriction during pregnancy affects maternal liver lipid metabolism and fetal brain lipid composition in the rat. Am J Physiol Endocrinol Metab. 2010; 298(2):E270-E7. doi: 10.1152/ajpendo.00437.2009.
Soares AKF, Guerra RGS, Castro ML, Amâncio-dosSantos A, Guedes RCA, Cabral-Filho JE, et al. Somatic and reflex development in suckling rats: Effects of mother treatment with ketogenic diet associated with lack of protein. Nutr Neurosci. 2009; 12(6):260-6. doi: 10.1179/147683009X423427.
Carlson SE, Carver JD, House SG. High fat diets varying in ratios of polyunsaturated to saturated fatty acid and linoleic to linolenic acid: A comparison of rat neural and red cell membrane phospholipids. J Nutr. 1986; 116(5):718-25.
Saste MD, Carver JD, Stockard JE, Benford VJ, Chen LiT, Phelps CP. Maternal diet fatty acid composition affects neurodevelopment in rat pups. J Nutr. 1998 [cited 2011 Jul 10]; 128:740-8. Available from: <http://jn.nutrition.org/content/128/4/740.full.pdf+html>.
Gajda AM, Michael MS, Pellizzon M, Ricci MR, Ulman EA. Diet induced metabolic syndrome in rodent models. Animal Lab News; 2007.
Farias HG. Considerações sobre dietas experimentais para animais de laboratório: formulações, aplicações, fornecimento e efeitos experimentais. I Simpósio de bioterismo da Fiocruz - PE; 2010 Out
; Recife, Brasil. Recife: Fiocruz; 2011. 16. Rolls BJ, Rowe EA, Fahrbach SE, Agius L, Williamson DH. Obesity and high energy diets reduce survival and growth rates of rat pups. Proc Nutr Soc. 1980; 39(2):51A.
Rolls BJ, Rowe EA. Pregnancy and lactation in the obese rat: Effects on maternal and pup weights. Physiol Behav. 1982; 28(3):393-400. doi: 10.1016/0031-9384(82)90130-5.
Instituto Brasileiro de Geografia e Estatística. Pesquisa de Orçamento Familiar, 2002- 2003: análise da disponibilidade domiciliar de alimentos e do estado nutricional no Brasil. Rio de Janeiro: IBGE; 2004.
Levy-Costa RB, Sichieri R, Pontes NS, Monteiro CA. Disponibilidade domiciliar de alimentos no Brasil: distribuição e evolução (1974-2003). Rev Saúde Pública. 2005; 39(4):530-40. doi: 10.1590/S0034-89102005000400003.
Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: some helpful considerations. Braz J Biol. 2002; 62(4):609-14. doi: 10.1590/S1519-69842002000400008.
Fishbeck KL, Rasmussen K. Effect of repeated reproductive cycles on maternal nutritional status, lactational performance and litter growth in ad libitum-fed and chronically food-restricted rats. J Nutr. 1987; 117:1967-75.
Ebisui L, Fontes, RS, Lapchik VBV. Rato. In: Lapchik VBV, Mattaraia VGM, KO GM. Cuidados e manejo de animais de laboratório. São Paulo: Atheneu; 2009.
Campbell JA. Method for determination of PER & NPR. In: Food and Nutrition Board: Evaluation of protein quality. Washington (DC): Food and Nutrition Board; 1963.
Fox WM. Reflex-ontogeny and behavioural development of the mouse. Anim Behav. 1965; 13(2): 234-41. doi: 10.1016/0003-3472(65)90041-2.
Shaw MA, Rasmussen KM, Myers TR. Consumption of a high fat diet impairs reproductive performance in Sprague-Dawley rats. J Nutr. 1997 [cited 2009 Feb 1]; 127(1):64-9. Available from: <http://jn.nutrition.org/content/127/1/64.long>.
Rasmussen KM. The influence of maternal nutrition on lactation. Annu Rev Nutr. 1992; 12:103-17. doi: 10.1146/annurev.nu.12.070192.000535.
Moretto VL, Ballen MO, Gonçalves TSS, Kawashita NH, Stoppiglia LF, Veloso RV, et al. Low-protein diet during lactation and maternal metabolism in rats. Obstet Gynecol. 2011; 2011:876502. doi: 10.5402/2011/876502.
Xu RY, WanYP, Tang QY, Wu J, Cai W. Carbohydrateto-fat ratio affects food intake and body weight in Wistar rats. Exp Biol Med. 2010; 235(7):833-8. doi: 10.1258/ebm.2010.009276.
Guo F, Jen K-LC. High-fat feeding during pregnancy and lactation affects offspring metabolism in rats. Physiol Behavior. 1995; 57(4):681-6. doi: 10.1016/0031-9384(94)00342-4.
Fernandes FS, Carmo MGT do, Herrera E. Influence of maternal diet during early pregnancy on the fatty acid profile in the fetus at late pregnancy in rats. Lipids. 2012; 47(5):505-17. doi: 10.1007/s11745-012-3660-7.
Ballen MLO, Moretto VL, Santos MP dos, Gonçalves TSS, Kawashita NH, Stoppigli LF, et al. Protein restriction in pregnancy: Effects related to dam metabolism. Arq Bras Endocrinol Metab 2009; 53(1):87-94. doi: 10.1590/S0004-27302009000100013.
Del Prado M, Delgado G, Vilalpando S. Maternal lipid intake during pregnancy and lactation alters milk composition and production and litter growth in rats. J Nutr. 1997 [cited 2011 Sept 18]; 127:458-62. Available from: <http://jn.nutrition.org/content/127/3/458.long>.
Marín MC, Alaniz MJT de. Relationship between dietary oil during gestation and lactation and biosynthesis of polyunsaturated fatty acids in
control and in malnourished dam and pup rats. J Nutr Biochem. 1998; 9(7):388-95.
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Derechos de autor 2023 Michelle Figueiredo CARVALHO, Meury Kétteryn Monteiro Evangelista da COSTA, Gisélia de Santana MUNIZ, Raul Manhães de CASTRO, Elizabeth do NASCIMENTO
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
