Evaluation of the biological quality of defatted pequi (Caryocar brasiliense Cambess) seed fl our protein supplemented with lysine to rats (Rattus norvegicus)
Palavras-chave:
Pequi seed, Protein quality, RatsResumo
Objective
In the biome of the Brazilian Cerrado, there are a lot of fruit tree species that stand out for their sensory quality and for presenting potentialities in the market of pulp and almond. Among these species, the pequi deserves attention because it has an almond rich in proteins and that is little explored. The aim of this study was to evaluate the biological quality of defatted pequi seed flour supplemented with lysine.
Methods
Two designs were done in this study; in the first, the animals were divided into four diet groups: control, proteinfree, defatted pequi seed flour and defatted pequi seed flour supplemented with lysine. The protein-free diet was exempt of proteins and the other diets had a protein content of 10% and differed in protein source (casein: control diet or defatted pequi seed flour: test diets). The experiment lasted for 14 days. In the second design, 36 animals were used and followed-up for 28 days. The division of the experimental groups was kept, except for the protein-free diet group, which was excluded. By the end of the test, the animals were anaesthetised and euthanized.
Results
The results showed that the protein efficiency ratio of the control group was significantly higher than the other groups. For the other indices, the groups that received defatted pequi seed flour did not differ statistically among themselves.
Conclusion
These findings have shown an effect of supplementation on the protein efficiency ratio when comparing the test diets, however, when compared to the control group, no improvement was found.
Referências
Fernandes DC, Freitas JB, Czeder LP, Naves MMV. Nutritional composition and protein value of the baru (Dipteryx alata Vog.) almond from the Brazilian Savanna. J Sci Food Agric. 2010;90(10):1650-5.
Sousa AGO, Fernandes DC, Naves MMV. Eficiência alimentar e qualidade proteica das sementes de baru e pequi procedentes do Cerrado brasileiro. Rev Inst Adolfo Lutz. 2012;71(2):274-80.
Alves AM, Fernandes DC, Sousa AGO, Naves RV, Naves MMV. Características físicas e nutricionais de pequis oriundos dos estados de Tocantins, Goiás e Minas Gerais. Braz J Food Technol. 2014;17(3):198-203.
Araújo FD. A review of Caryocar brasiliense (Caryocaracea): An economically valuable species of the Central Brazilian Cerrados. Econ Botany.1995;40(1):40-8.
Damiani C, Almeida TL, Costa NV, Medeiros NX, Silva AGM, Silva FA, et al. Perfil de ácidos graxos e fatores antinutricionais de amêndoas de pequi crua e torrada. Pesq Agropec Trop. 2013;43(1):71-8.
Silva MAP, Medeiros Filho S. Morfologia de fruto, semente e plântula de piqui (Caryocar coriaceum Wittm.). Rev Ciênc Agron. 2006;37(3):320-5.
Ramos KMC, Souza VAB. Características físicas e químico-nutricionais de frutos de pequizeiro (Caryocar coriaceum Wittm.) em populações naturais da região meio-norte do Brasil. Rev Bras Frutic. 2011;33(2):500-8.
Cordeiro MWS, Cavallieri ALF, Ferri PH, Naves MMV. Características físicas, composição químico-nutricional e dos óleos essenciais da polpa de Caryocar brasiliense nativo do estado de Mato Grosso. Rev Bras Frutic. 2013;35(4):1127-39.
Lima A, Silva AMO, Trindade RA, Torres RP, Mancini-Filho J. Composição química e compostos bioativos presentes na polpa e na amêndoa do pequi (Caryocar brasiliense, Camb.). Rev Bras Frutic. 2007;29(3):695-8.
Teixeira TN, Esteves EA, Oliveira LG, Oliveira MLP, Santana RC, Dessimoni NAV, et al. Caryocar brasiliense pulp increases serum HDL and reduces hepatic lipid accumulation in rats fed a high fat diet. J Med Plants Res. 2013;7(15):963-9.
Oliveira MEB, Guerra NB, Barros LM, Alves RE. Aspectos agronômicos e de qualidade do pequi. Brasília: Embrapa; 2008.
Venkatachalam M, Sathe SK. Chemical composition of selected edible nut seeds. J Agric Food Chem. 2006;54(13):4705-14.
Cândido PA, Malafaia GC, Rezende ML. A exploração do pequi na região norte de Minas Gerais: abordagem por meio do sistema agroalimentar localizado. Rev Ideas. 2012;5(2):118-38.
Oliveira WL, Scariot A. Boas práticas de manejo para o extrativismo sustentável do pequi. Brasília: Embrapa Genetic Resources and Biotechnology; 2010.
Oliveira MEB, Guerra NB, Maia AHN, Alves RE, Matos NMS, Sampaio FGM. Características químicas e físico--químicas de pequis da Chapada do Araripe, Ceará. Rev Bras Frutic. 2010;32(1):114-25.
Roesler R, Malta LG, Carrasco LC, Holanda RB, Sousa CAS, Pastore GM. Atividade antioxidante de frutas do cerrado. Ciênc Tecnol Aliment. 2007;27(1):53-60.
Woolf PJ, Fu LL, Basu A. vProtein: Identifying optimal amino acid complements from plant-based foods. Plos One. 2011;6(4):e18836. http://dx.doi.org/10.1371/journal.pone.0018836
Molina SMG, Gaziola SA, Lea PJ, Azevedo RA. Manipulação de cereais para acúmulo de lisina em sementes. Scientia Agric. 2001;58(1):205-11.
Alves LF, Rocha MS, Gomes CCF. Avaliação da qualidade protéica da Quinua Real (Chenopodium quinoa Willd) através de métodos biológicos. e-Scientia. 2008;1(1):1-16.
Arentson-Lantz E, Clairmont S, Paddon-Jones D, Tremblay A, Elango R. Protein: A nutrient in focus. Appl Physiol Nutr Metab. 2015;40(8):755-61.
Nutten S. Proteins, peptides and amino acids: role in infant nutrition. In: Bhatia J, Shamir R, Vandenplas Y. Protein in neonatal and infant nutrition: Recent updates. 86th ed. Beijing: Nestle Nutr Inst Workshop; 2016. p.1-10.
Millward DJ, Layman DK, Tomé D, Schaafsma G. Protein quality assessment: Impact of expanding understanding of protein and amino acid needs for optimal health. Am J Clin Nutr. 2008;87(5):1576-81.
Schaafsma G. Advantages and limitations of the protein digestibility-corrected amino acid score (PDCAAS) as a method for evaluating protein quality in human diets. Br J Nutr. 2012;108(Suppl.2):333-6.
Wolfe RR. Update on protein intake: Importance of milk proteins for health status of the elderly. Nutr Rev. 2015;73(Suppl.1):41-7.
Osborne TB, Mendel LB, Ferry EL. A method of expressing numerically the growth-promoting value of proteins. J Biol Chem. 1919;37:223-9.
Angelis RC. Valor nutricional das proteínas: métodos de avaliação. Cad Nutr Soc Bras Alim Nutr. 1995;10(1):8-29.
Bender AE, Doel BH. Biological evaluation of proteins: A new aspect. Br J Nutr. 1957;11(2):140-8.
Mitchell GV, Jenkins MY, Grundel E. Protein efficiency ratios and net protein rations of selected protein foods. Plant Food Hum Nutr. 1989;39(1):53-8.
Gilani GS, Xiao CW, Cockell KA. Impact of antinutritional factors in food proteins on the digestibility of protein and the bioavailability of amino acids and on protein quality. Br J Nutr. 2012;108(Suppl.2):S315-32.
Gilani GS, Cockell KA, Sepehr E. Effects of antinutritional factors on protein digestibility and amino acid availability in foods. J AOAC Inter. 2005;88(3):966-87.
Miller DS, Bender AE. The determination of the net utilization of proteins by a shortened method. Br J Nutr. 1955;9(4):382-8.
Pellet PL, Kaba H. Carcass amino acids of the rat under conditions of determination of the net protein utilization. J Nutr. 1972;102(1):61-8.
Lachance PA, Miller GA. Protein quality assessment in the rat: Correlation between whole carcass and hind limb nitrogen concentration. Nutr Rep Int. 1973;7(1):25-31.
Instituto Adolfo Lutz. Métodos físico-químicos para análise de alimentos. São Paulo: IAL; 2008. E-book.
Association of Official Analytical Chemists. (986.08) (985.29) (960.52): Official methods of analysis of the Association of Official Analytical Chemists. 16th ed. Washington (DC): AOAC; 1997.
Spies JR. Determination of tryptophan in proteins. J Anal Chem. 1967;39(12):1412-5.
White JA, Hart RJ, Fry JC. An evaluation of the Waters Pico-Tag system for the amino-acid analysis of food materials. J Autom Chem. 1986;8(4):170-7.
Hagen SR, Frost B, Augustin J. Precolumn phenylisothiocyanate derivatization and liquid chromatography of amino acids in food. J Assoc Off Anal Chem. 1989;72(6):912-6.
Association of Official Analytical Chemists. (39.133): Official methods of analysis of the Association of Official Analytical Chemists.10th ed. Washington (DC): AOAC; 1965.
Food and Agriculture Organization. Energy and protein requirements: Report of a joint FAO/WHO/UNU expert consultation meeting. Geneva: WHO; 1985.
Sousa AGO, Fernandes DC, Alves AM, Freitas JB, Naves MMV. Nutritional quality and protein value of exotic almonds and nut from the Brazilian Savanna compared to peanut. Food Res Inter. 2011;44(7):2319-25.
Freitas JB, Naves MMV. Composição química de nozes e sementes comestíveis e sua relação com a nutrição e saúde. Rev Nutr. 2010;23(2):269-79. http://dx.doi.org/10.1590/S1415-52732010000200010
Institute of Medicine. Dietary reference intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. Washington (DC): National Academy Press; 2002 [cited 2017 Apr 7]. Available from: https://www.nap.edu
Institute of Medicine. Dietary reference intakes for water, potassium, sodium, chloride, and sulfate. Washington (DC): National Academy Press; 2004 [cited 2017 Apr 7]. Available from: https://www.nap.edu45. Padovani RM, Amaya-Farfán J, Colugnati FAB, Domene SMA. Dietary reference intakes: aplicabilidade das tabelas em estudos nutricionais. Rev Nutr. 2006;19(6):741-60. http://dx.doi.org/10.1590/S1415-527
Mao X, Hua Y, Chen G. Amino acid composition, molecular weight distribution and gel electrophoresis of walnut (Juglans regia L.) proteins and protein fractionations. Int J Mol Sci. 2014:15(2):2003-14.
Food and Agriculture Organization. Protein quality evaluation: United Nations Report of a joint FAO/WHO expert consultation. Rome: FAO; 1991.
Pires CV, Oliveira MGA, Rosa JC, Costa NMB. Qualidade nutricional e escore químico de aminoácidos de diferentes fontes protéicas. Ciênc Tecnol Aliment. 2006;26(1):179-87.
Hiane PA, Macedo MLR, Silva GM, Braga Neto JA. Avaliação nutricional da proteína de amêndoas de bocaiúva, Acrocomia aculeata (Jacq.) Lodd, em ratos Wistar em crescimento. Bol Ceppa. 2006;24(1):191-206.
Lujan DLB, Leonel AJ, Bassinello PZ, Costa NMB. Variedades de feijão e seus efeitos na qualidade protéica, na glicemia e nos lipídios sanguíneos em ratos. Ciênc Tecnol Aliment. 2008;28:142-9.
Tomé D. Digestibility issues of vegetable versus animal proteins: Protein and amino acid requirements: Functional aspects. Food Nutr Bull. 2013;34(2):272-4.
Monteiro MRP, Costa NMB, Oliveira MGA, Pires CV, Moreira MA. Qualidade protéica de linhagens de soja com ausência do Inibidor de Tripsina Kunitz e das isoenzimas Lipoxigenases. Rev Nutr. 2004;17(2):195-205. http://dx.doi.org/10.1590/S1415-52732004000200006
Souza JC, Fernandes DC, Naves MMV. Qualidade protéica de multimisturas distribuídas em Alfenas, Minas Gerais, Brasil. Rev Nutr. 2006;19(6):685-92. http://dx.doi.org/10.1590/S1415-52732006000600005
Monteiro MRP, Martino HSD. Avaliação nutricional e sensorial do extrato hidrossolúvel de soja. Rev Min Enf. 2006;10(2):113-7.
Roman JA, Sgarbieri VC. Obtenção e caracterização química e nutricional de diferentes concentrados de caseína. Rev Nutr. 2005;18(1):75-83. http://dx.doi.org/10.1590/S1415-52732005000100007
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Copyright (c) 2023 Miliane Martins de Andrade FAGUNDES, Ana Maria Fernandes VIANA, Mayara Medeiros de Freitas CARVALHO, Marcelo Eustáquio SILVA
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