Cytokine profile of rats fed a diet containing shrimp
Palabras clave:
Cytokines, Diet, Eosinophils, InterleukinsResumen
Objective
Studies have shown that shrimps reduced the tensile strength of scars in rat skin. The aim of the present study was to assess the cytokine profile of rats fed shrimp.
Methods
Group 1 (control) received a regular diet and Group 2 (experimental) received a diet containing 33% shrimp for nine days. The two diets contained the same amounts of proteins, fats and carbohydrates. Serum cytokine levels were determined by ELISA and a segment of the jejunum was taken to investigate its histological morphology and eosinophil infiltrate.
Results
The experimental group had lower serum levels of interleukin-4 (IL-4) (14.4±1.9 versus 18.11±2.6pg/mL; p<0.05) and IL-10 (5.0±0.98 versus 7.5±1.2pg/mL; p<0.05) and higher levels of IL-6 (17.8±2.3 versus 3.2±0.4pg/mL, p<0.001) than controls. Morphologically, the shrimp-based diet caused an architectural disorganization of the intestinal mucosa and a greater amount of eosinophils in the jejunal villus.
Conclusion
Our data suggests that shrimp consumption leads to a significant increase in the cytokine IL-6, a decrease in the immunomodulatory cytokine IL-10 in the serum of rats, and high eosinophil infiltration in the jejunum. The cytokine profile typical of inflammation and the histological aspect of the jejunum are compatible with food allergy.
Citas
Cribbs RK, Harding PA, Luquette MH, Besner GE. Endogenous production of heparin-like EGF-like growth factor during murine partial-thickness burn wound healing. J Burn Care Rehabil. 2002; 23(2): 116-25.
Crowe MJ, Doestschman T, Greenhalgh DG. Delayed wound healing in immunudeficient TGF-beta 1 knockout mice. J Invest Dermatol. 2000; 115(1):3-11.
Dammeier J, Beer HD, Brauchle M, Werner S. Dexamethasone is a novel potent inducer of connective tissue growth factor expression: implications for glucocorticoid therapy. J Biol Chem. 1998; 273(29):18185-90. doi: 10.1074/jbc.273.29.18185.
Danilenko DM, Ring BD, Lu JZ, Tarpley JE, Chang D, Liu N, et al. New differentiation factor upregulates epidermal migration and integrin expression in excisional wounds. J Clin Invest. 1995; 95(2):842-51.
Danilenko DM, Ring BD, Tarpley JE, Morris B, Van GY, Morawiecki A, et al. Growth factoring porcine full and partial thickness burn repair. Differing targets and effects of keratinocyte growth factor, platelet derived growth factor-BB, epidermal growth factor, and neu differentiation factor. Am J Pathol. 1995; 147(5):1261-77.
Diehl S, Rincón M. The two faces of IL-6 on Th1/Th2 differentiation. Mol Immunol. 2002; 39(9):531-6.
Bundoc VG, Keane-Myers A. IL-10 confers protection from mast cell degranulation in a mouse model of allergic conjunctivitis. Exp Eye Res. 2007; 85(4):575-9.
Koulis A, Robinson DS. The anti-inflammatory effects of interleukin-10 in allergic disease. Clin Exp Allergy. 2000; 30(6):747-50.
Tournoy KG, Kips JC, Pauwels RA. Endogenous interleukin-10 suppresses. Allergy. 2000; 30(6): 775-83.
Knippels LMJ, Wijk FW, Penninks AH. Food allergy: what do we learn from animal models? Curr Opin Allerg Clin Immunol. 2004; 4(3):205-9.
Ogawa T, Miura S, Tsuzuki Y, Ogino T, Teramoto K, Inamura T, et al. Chronic allergy to dietary ovalbumin induces lymphocyte migration to rat small intestinal mucosa that is inhibited by MAdCAM-1. Am J Physiol Gastrointest Liver Physiol. 2004; 286(5):G702-10.
Borges EL, Pereira FKS, Alvarez-Leite JI, Alberti LR, Ferreira MAND, Petroianu A. Shrimp diet and skin healing strength in rats. Rev Nutr. 2007; 20(3):257-63. doi: 10.1590/S141552732007000300004.
Lowry OH, Rosenbrough NJ, Farr AL, Randal AL. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75.
Ahern DJ, Robinson DS. Regulatory T cells as a target for induction of immune tolerance in allergy. Curr Opin Allergy Clin Immunol. 2005; 5(6):531-6.
Doganci A, Eigenbrod T, Krug N, De Sanctis GT, Hausding M, Erpenbeck VJ, et al. The IL-6RD chain controls lung CD4+CD25+ Treg development and function during allergic airway inflammation in vivo. J Clin Invest. 2005; 115(2):313-25.
Sato Y, Ohshima T, Kondo T. Regulatory role of endogenous interleukin-10 in cutaneous inflammatory response of murine wound healing. Biochem Biophys Res Commun. 1999; 265(1):194-9.
Zubaidi A, Buie WD, Hart DA, Sigalet D. Temporal expression of cytokines in rat cutaneous, fascial, and intestinal wounds: a comparative study. Dig Dis Sci. 2010; 55(6):1581-8.
Prigione I, Morandi F, Tosca MA, Silvestri M, Pistoia V, Ciprandi G, et al. Interferon-gamma and IL-10 may protect from allergic polysensitization in children: preliminary evidence. Allergy. 2010; 65(6):740-2.
Chuang YH, Yang YH, Wu SJ, Chiang BL. Gene therapy for allergic diseases. Curr Gen Ther. 2009; 9(3):185-91.
Tay SS, Plain KM, Bishop GA. Role of IL-4 and Th2 responses in allograft rejection on tolerance. Curr Opin Organ Transplant. 2009; 14(1):16-22.
Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev. 2003; 83(3):835-70.
Hübner G, Brauchle M, Smola H, Madlener M, Fassler R, Werner S. Differential regulation of pro-inflammatory cytokines during wound healing in normal and glucocorticoid-treated mice. Cytokine. 1996; 8(7):548-56.
Feiken E, Romer J, Eriksen J, Lund LR. Neutrophils express tumor necrosis factor-alpha during mouse skin wound healing. J Invest Dermatol. 1995; 105(1): 120-3.
Gallucci RM, Simeonova PP, Matheson JM, Kommineni C, Guriel JL, Sugawara T, et al. Impaired cutaneous wound healing in interleukin-6-deficient and immunosuppressed mice. FASEB J. 14(15): 2525-31.
Sato M, Sawamura D, Ina S, Yaguchi T, Hanada K, Hashimoto I. In vivo introduction of the interleukin 6 gene into human keratinocytes: induction of epidermal proliferation by the fully spliced form of interleukin 6, but not by the alternatively spliced
form. Arch Dermatol Res. 1999; 291(7-8):400-4. doi: 10.1007/s004030050429.
Moore KW, De Waal Malefyt R, Coffman RL, O’Garra A. Interleukin-10 and interleukin-10 receptor. Annu Rev Immunol. 2001; 19:683-765.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2023 Elizabeth Lage BORGES, Dirce Ribeiro de OLIVEIRA, Lucíola da Silva BARCELOS, Jorge Luiz PESQUERO
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
